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KMID : 0358320060470040418
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Korean Journal of Urology
2006 Volume.47 No. 4 p.418 ~ p.425
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The Inhibition of Human Telomerase Reverse Transcriptase Expression by Peroxiredoxin I and c-Myc in Prostatic Cancer Cells
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Chang In-Ho
Kim Hwa-Su
Kim Tae-Hyoung
Myung Soon-Chul
Lee Sang-Eun
Kim Young-Sun
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Abstract
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Purpose: We evaluated the hypothesis that the telomerase expression is associated with c-Myc and peroxiredoxin I(Prx I) in patients with prostate cancer. The study determined the link between Prx I, c-Myc and human telomerase reverse transcriptase(hTERT) in prostate cancer cells.
Materials and Methods: The cDNA of the Prx I gene was obtained by reverse-transcriptase polymerase chain reaction(RT-PCR) amplification. Cotransfections were performed by using a hTERT luciferase reporter plasmid and each expression vector as indicated(c-Myc or Prx I). Empty vectors were used as controls for determining the basal promoter activity. RT-PCR was performed to evaluate the effect of the DEM-induced Prx I mRNA expression. Luciferase assay was performed to evaluate the inhibitory effect of transfected Prx I and the DEM induced Prx I on the transcriptional activity of hTERT in the human prostatic cancer cell lines PC-3 and DU-145.
Results: In this study, we found that Prx I could inhibit hTERT expression through direct interaction with c-Myc protein in the prostate cancer cell lines. In addition, it was obvious that Prx I could interact with c-Myc protein. We also found that DEM could induce upregulation of the Prx I mRNA expression and that the increased expression of Prx I could downregulate the expression of hTERT.
Conclusions: Our results demonstrated a direct link between Prx I, c-Myc and hTERT, and we suggest that Prx I regulates cellular immortalization through c-Myc and hTERT, which is activation step in carcinogenesis. (Korean J Urol 2006;47:418-425)
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KEYWORD
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Telomerase reverse transcriptase, Peroxiredoxin I, Genes, myc, Prostate cancer
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